She acquired the Roydon Estates and other properties, and, through her son, Roger (H3), succeeded in her determination to establish the Twysdens as heirs of Roydon.
Her daughter, Margaret, born 1545, married Richard Dering of Pluckley, Kent.
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H2 (215). Margaret Twysden. 1564-1630.
Portrait, in black dress with puffed and slashed sleeves, with elaborate white collar and ruffles, and holding fan.
Date: Unknown. (29" X 24".) Artist: possibly Gilbert Jackson.
Daughter of Roger Twysden (H3), grand-daughter of Elizabeth Roydon (H1).
Married: Henry Fane, Esq., of Fairlawn, Kent. Their grandson, Sir Harry Vane, the younger, was the prominent Cavalier.
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H3 (211). Roger Twysden. 1542-1603. Plate III.
Portrait, in black with white ruff. The crest, a cockatrice, and the Arms of Twysden -- quartering Roydon --- with the motto Previsa pereunt mala are depicted in top left corner.

Circa 1587. (44 1/2" X 32".) Artist: Unknown.
Son of Elizabeth Roydon (H1) and William Twysden of Wye and Chelmington, who married in 1539.
Married: Anne Wiat, daughter of Sir Thomas Wiat, the younger, of Allington Castle, Kent, 1562.
He consolidated the estates and the position of the family, and was Sheriff of Kent, 1599.
He lived at Roydon Hall, East Peckham, Kent, and was succeeded by his son, William (H4).
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H4 (263). Sir William Twysden, 1st Baronet of Roydon Hall. 1566-1629. Plate V.
Portrait, in black doublet and gown, with ruff, cuffs and sword belt, and holding a walking stick.
Dated 1629. (29 3/4" X 23 3/4".) Artist: Unknown.
Son of Roger Twysden (H3) and Anne Wiat (D9).
Married: Anne Finch (H5), daughter of Sir Moyle Finch, 1st Bart. of Eastwell, Kent (Hm1) by Elizabeth, 1st Countess of Winchelsea (Hc).
He was a Member of Parliament and at the Court of Queen Elizabeth. He took part, with Essex, Howard and Ralegh, in an expedition to intercept the Spanish West Indian Fleet, 1596-7